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KMID : 0613820070170111555
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Journal of Life Science
2007 Volume.17 No. 11 p.1555 ~ p.1561
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Effects of Biologically Active Materials Prepared for Several Minerals and Plants on the Growth of Rumen Microbes
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Lee Shin-Ja
Ok J. U.
Lee Sang-Min
Lim J.-H.
Kim Kyoung-Hoon
Moon Yea-Hwang
Lee Sung-Sill
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Abstract
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In order to know the effects of scoria, germanium, charcoal, ginger, stevia, and CLA(Conjugated Linoleic Acid) as biologically active materials on pathogenic microbes and rumen anaerobic microbes, the growth rate of pathogens (including Escherichia coli O157, Salmonella paratyphi, Listeria monocytogenes and Staphylococcus aureus) and in vitro rumen microbial growth, gas production, ammonia concentration, carboxymethyl-cellulase (CMCase) activity, and microbial populations were investigated. The growth of pathogenic microbes was inhibited by the supplement of 0.10% ginger. Ginger had powerful antimicrobial properties on all the pathogens used in this experiments. Additionally in the antibacterial assay by paper disc method, we could observe the clear zone of similar area with the positive control(antibiotics) for E. coli as applied with the 10% stevia or the 10% CLA only. The supplements of ginger, stevia and CLA in in vitro rumen fermentation inhibited populations of rumen bacteria and protozoa. Particularly supplement of ginger resulted in remarkable reduction of the protozoa population, which means it might serve as a source inhibiting material of methane creation in the rumen.
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KEYWORD
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Biologically active materials, pathogenics, rumen microbes, microbial growth
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